ISSN: 2251-8363    eISSN: 2251-8819  
J Nephropathol. 2012;1(2):91-100.
doi:10.5812/nephropathol.7518
PMID: 24475396
PMCID: PMC3886135

Scopus id: 84867245474

Original Article

Detection of immunoglobulins and complement components in formalin fixed and paraffin embedded renal biopsy material by immunoflourescence technique

Muhammed Mubarak 1 * , Javed I Kazi 1, Umme Kulsoom 1, Muhammed Ishaque 1

1 Histopathology Department, Sindh Institute of Urology and Transplantation, Karachi, Pakistan.
*Corresponding author: Dr. Muhammed Mubarak, Histopathology Department, Sindh Institute of Urology and Transplantation, Karachi-74200, Pakistan. Telephone: 009221 99215752, Fax: 009221 32726165, Email: drmubaraksiut@yahoo.com

Abstract

Background: The technique of direct immunoflourescence (IF) is essential in the accurate diagnosis of renal glomerular diseases. The optimal results are obtained when the procedure is done on fresh frozen tissue (IF-F). However, techniques are available for IF study on formalin fixed and paraffin embedded (FFPE) renal biopsy specimens with variable reported success rates.

Objectives: We evaluated three such techniques on FFPE tissue and compared the results with those obtained by IF-F from the same patients.

Materials and Methods: Heat treatment with Tris buffer and citrate buffer, and pronase treatment of the FFPE material was carried out. Direct IF was done for renal panel immunoglobulins and complement components on all biopsies and the results were compared with the historical IF-F study.

Results: When compared to the IF-F, the immunoflourescence staining on the paraffin sections was less sensitive and less intense in all immune complex-mediated renal diseases, but the diagnostic findings were detected in majority of the cases.

Conclusions: In conclusion, it is possible to establish the diagnosis in most cases of immune complex-mediated glomerular diseases with IF on paraffin embedded tissue specimens.

Implication for health policy/practice/research/medical education:

Heat treatment and proteolytic digestion of formalin fixed and paraffin embedded renal tissue sections are valuable salvage techniques for immunoflourescence study, if frozen tissue is not available. These methods can be utilized in the laboratories which lack the facilities for procurement of fresh frozen renal biopsies.

Please cite this paper as: Mubarak M, Kazi JI, Kulsoom U, Ishaque M. Detection of immunoglobulins and complement components in formalin fixed and paraffin embedded renal biopsy material by immunoflourescence technique. J Nephropathology. 2012; 1(2): 91-100. DOI: 10.5812/nephropathol.7518

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First published online: 01 Jul 2012
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